1BFO

CAMPATH-1G IGG2B RAT MONOCLONAL FAB

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	94.280, 108.150, 166.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.192
Rwork	0.192
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIALLY REFINED CAMPATH-1H
RMSD bond length	0.017
RMSD bond angle	26.400 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	CNS (0.3)
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.077	0.327 *
Number of reflections	47690
<I/σ(I)>	5	1.5
Completeness [%]	90.5	78.6
Redundancy	2.1 *	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5		pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG10000	20 (%)
3	1	reservoir	imidazole/malate	0.2 (M)