1BFN

BETA-AMYLASE/BETA-CYCLODEXTRIN COMPLEX

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-11
Detector	RIGAKU
Spacegroup name	P 31 2 1
Unit cell lengths	86.030, 86.030, 144.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.070
R-factor	0.158
Rwork	0.158
R-free	0.21100
RMSD bond length	0.013
RMSD bond angle	24.300 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.070 *
Rmerge	0.060 *
Total number of observations	152726 *
Number of reflections	36696 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.4	4 *	49% (NH4)2SO4, 0.1M NAOAC PH 5.4, 18MM 2-MERCAPTOETHANOL, AT 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	ammonium sulfate	24.5 (%)
3	1	drop	sodium acetate	0.05 (M)
4	1	drop	EDTA	0.5 (mM)
5	1	drop	ME	9 (mM)
6	1	reservoir	ammonium sulfate	49 (%)
7	1	reservoir	sodium acetate	0.1 (M)
8	1	reservoir	EDTA	1 (mM)
9	1	reservoir		18 (mM)