1BFE

THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01
Detector	MARRESEARCH
Spacegroup name	P 41 3 2
Unit cell lengths	89.200, 89.200, 89.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.300
R-factor	0.214 *
Rwork	0.214
R-free	0.31500
Structure solution method	MIR
RMSD bond length	0.013 *
RMSD bond angle	1.800 *
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.380
High resolution limit [Å]	2.200	2.300
Rmerge	0.036	0.166
Total number of observations	38267 *
Number of reflections	6421
Completeness [%]	96.6	90.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		0.8 M SODIUM CITRATE, 0.1 M HEPES, PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.8 (M)
2	1	reservoir	HEPES	0.1 (M)	pH7.5
3	1	drop	protein	15 (mg/ml)