1BFC

BASIC FIBROBLAST GROWTH FACTOR COMPLEXED WITH HEPARIN HEXAMER FRAGMENT

Experimental procedure

Detector technology	AREA DETECTOR
Spacegroup name	P 21 21 21
Unit cell lengths	33.150, 41.800, 85.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.188
Rwork	0.188
RMSD bond length	0.150
RMSD bond angle	2.370
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.200
Rmerge	0.073 *
Number of reflections	6123
Completeness [%]	94.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	heparin tetrasaccharide	10 (mg/ml)
2	1	drop	bFGF	10 (mg/ml)
3	1	reservoir	PEG8000	34 (%)
4	1	reservoir		30 (mM)
5	1	reservoir	N-[2-acetamido]-2-iminodiacetic acid	0.1 (M)