1BFA

RECOMBINANT BIFUNCTIONAL HAGEMAN FACTOR/AMYLASE INHIBITOR FROM MAIZE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-10
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 42 21 2
Unit cell lengths	57.120, 57.120, 80.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.205
Rwork	0.205
R-free	0.26700
Structure solution method	DIRECT PHASING
Starting model (for MR)	1CBA
RMSD bond length	0.007
RMSD bond angle	22.100 *
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.200	2.200
Number of reflections	4996
Completeness [%]	77.0	45.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *		PROTEIN WAS CRYSTALLIZED FROM 10MG/ML PROTEIN, 1% 2-PROPANOL, 1% PEG-4000, 0.1 M SODIUM CITRATE PH 5.4.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir	PEG400	30 (%)
3	1	reservoir		0.2 (M)
4	1	reservoir	HEPES	0.1 (M)	pH7.5