1BF4
CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-15 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.870, 49.670, 37.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.600 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SSO7D-GTAATTAC |
| RMSD bond length | 0.012 |
| RMSD bond angle | 24.000 * |
| Data reduction software | bioteX (V. 1.1) |
| Data scaling software | bioteX (V. 1.1) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.840) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.074 | 0.278 * |
| Number of reflections | 11595 | |
| <I/σ(I)> | 15 | 2.4 |
| Completeness [%] | 95.1 | 86.6 |
| Redundancy | 4.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Sso7d | 1.3 (mM) | |
| 2 | 1 | drop | DNA duplex | 1.3 (mM) | |
| 3 | 1 | drop | Tris-Cl | 2 (mM) | |
| 4 | 1 | drop | MPD | 5 (%) | |
| 5 | 1 | drop | PEG400 | 2.6 (%) | |
| 6 | 1 | reservoir | MPD | 20 (%) |
