1BF4
CHROMOSOMAL DNA-BINDING PROTEIN SSO7D/D(GCGAACGC) COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 123 |
Detector technology | IMAGE PLATE |
Collection date | 1997-07-15 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.870, 49.670, 37.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.600 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SSO7D-GTAATTAC |
RMSD bond length | 0.012 |
RMSD bond angle | 24.000 * |
Data reduction software | bioteX (V. 1.1) |
Data scaling software | bioteX (V. 1.1) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.840) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.074 | 0.278 * |
Number of reflections | 11595 | |
<I/σ(I)> | 15 | 2.4 |
Completeness [%] | 95.1 | 86.6 |
Redundancy | 4.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Sso7d | 1.3 (mM) | |
2 | 1 | drop | DNA duplex | 1.3 (mM) | |
3 | 1 | drop | Tris-Cl | 2 (mM) | |
4 | 1 | drop | MPD | 5 (%) | |
5 | 1 | drop | PEG400 | 2.6 (%) | |
6 | 1 | reservoir | MPD | 20 (%) |