1BEZ
HALOALKANE DEHALOGENASE MUTANT WITH TRP 175 REPLACED BY TYR AT PH 5
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 120 |
Detector technology | DIFFRACTOMETER |
Collection date | 1995-09-11 |
Detector | ENRAF-NONIUS FAST |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.030, 72.300, 40.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.171 |
Rwork | 0.171 |
R-free | 0.22500 |
Starting model (for MR) | 1bee |
RMSD bond length | 0.008 |
RMSD bond angle | 23.400 * |
Data reduction software | MADNES |
Data scaling software | BIOMOL |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 2.130 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.075 * | 0.136 * |
Total number of observations | 65239 * | |
Number of reflections | 13700 | |
<I/σ(I)> | 14 | |
Completeness [%] | 79.0 | 60.5 * |
Redundancy | 4.8 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 | PROTEIN WAS CRYSTALLIZED FROM 50% AMMONIUM SULFATE, 100 MM MES, PH 5.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.5 (mg/ml) | |
2 | 1 | drop | MES | 100 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 50 (%) | |
4 | 1 | reservoir | MES | 100 (mM) |