1BEO
BETA-CRYPTOGEIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 46.510, 46.510, 134.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.200 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27900 |
| Structure solution method | MAD+NATIVE DATA |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.200 * | |
| Rmerge | 0.032 * | 0.150 * |
| Total number of observations | 35101 * | |
| Number of reflections | 7864 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 75 (mg/ml) | |
| 2 | 1 | drop | 0.02 (%) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | 4.85 (M) | ||
| 5 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) | can be replaced by 50mM CAPS |
