1BEH
HUMAN PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN IN COMPLEX WITH CACODYLATE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.430, 61.630, 67.920 |
Unit cell angles | 90.00, 101.09, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.235 * |
Rwork | 0.235 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bd9 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.025 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.055 | 0.280 |
Number of reflections | 38803 | |
<I/σ(I)> | 16.2 | 2.6 |
Completeness [%] | 95.6 | 94.9 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 28-32% PEG 4000/6000/8000, 200-300 MM SODIUM ACETATE, 100MM SODIUM CACODYLATE., pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 5 (mg/ml) | can be replaced by 10mg/ml |
2 | 1 | 1 | Bis-Tris | 5 (mM) | |
3 | 1 | 1 | sodium azide | 0.01 (%) | |
4 | 1 | 2 | PEG4000 | 28-32 (%) | can be replaced by PEG6000 or PEG8000 |
5 | 1 | 2 | sodium acetate | 200-300 (mM) | |
6 | 1 | 2 | sodium azide | 0.01 (%) | |
7 | 1 | 2 | sodium cacodylate | 100 (mM) |