1BEB
BOVINE BETA-LACTOGLOBULIN, LATTICE X
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1988-09 |
| Detector | XENTRONICS |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.800, 49.500, 56.600 |
| Unit cell angles | 123.40, 97.30, 103.70 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | B-LG LATTICE Z - SOLVED BY MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.084 * |
| Data reduction software | ROTAVATA |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.960 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.070 |
| Number of reflections | 25155 | |
| <I/σ(I)> | 5 | 3 |
| Completeness [%] | 97.0 | 78 |
| Redundancy | 2.1 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch method | 6.5 | 25MG/ML PROTEIN WAS DISSOLVED IN 0.1M SODIUM/ POTASSIUM PHOSPHATE BUFFER (PH 7.0) AND CRYSTALLIZED BY THE BATCH METHOD FROM 2M AMMONIUM SULFATE (PH 6.5) AND 0.4M SODIUM/POTASSIUM PHOSPHATE BUFFER (PH 7.6)., batch method |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 22.7 (mg/ml) | |
| 2 | 1 | 1 | sodium potassium phosphate | 0.42 (M) | |
| 3 | 1 | 1 | ammonium sulfate | 2 (M) | adjuste to pH6.5 with 0.88M ammonia |
| 4 | 1 | 1 | water | 0.25ml | |
| 5 | 1 | 1 | sodium azide | 0.001 (%(w/v)) |
