1BE9

THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95 IN COMPLEX WITH A C-TERMINAL PEPTIDE DERIVED FROM CRIPT.

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01
Detector	MARRESEARCH
Spacegroup name	P 41 3 2
Unit cell lengths	89.340, 89.340, 89.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.820
R-factor	0.207 *
Rwork	0.207
R-free	0.27800
Structure solution method	DIFFERENCE FOURIER ANALYSIS
RMSD bond length	0.013 *
RMSD bond angle	1.700 *
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.880
High resolution limit [Å]	1.820	1.820
Rmerge	0.048	0.144
Total number of observations	141652 *
Number of reflections	11108
Completeness [%]	95.7	90.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5		0.8 M SODIUM CITRATE, 0.1 M HEPES, PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.8 (M)
2	1	reservoir	HEPES	0.1 (M)	pH7.5
3	1	drop	protein	15 (mg/ml)