1BDV
ARC FV10 COCRYSTAL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.840, 56.310, 52.980 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.252 * |
| Rwork | 0.243 |
| R-free | 0.29700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1par |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.573 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.800 | 2.500 |
| Rmerge | 0.081 | |
| Number of reflections | 8691 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 97.5 | 62 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | pH 6.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | GLYCEROL | ||
| 3 | 1 | 1 | CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | COBALTHEXAMMINE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium phosphate | 55 (%sat) |
