1BDH
PURINE REPRESSOR MUTANT-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Detector technology | AREA DETECTOR |
Collection date | 1995-11-22 |
Detector | UCSD MARK III |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 176.060, 95.490, 80.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.700 |
R-factor | 0.168 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.900 |
Data reduction software | UCSD |
Refinement software | TNT |
Data quality characteristics
Overall | |
Rmerge | 0.067 |
Number of reflections | 25422 |
Completeness [%] | 98.0 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.12 (mM) | |
10 | 1 | reservoir | PEG4000 | 25 (%) | |
11 | 1 | reservoir | ammonium sulphate | 0.4 (M) | |
12 | 1 | reservoir | cobalt hexammine | 50 (mM) | |
13 | 1 | reservoir | ammonium phosphate | 0.1 (M) | |
2 | 1 | drop | dithiothreitol | 0.2 (mM) | |
3 | 1 | drop | sodium-potassium phosphate | 38 (mM) | |
4 | 1 | drop | DNA | 1.7 (mM) | |
5 | 1 | drop | sodium cacodylate | 14.3 (mM) | |
6 | 1 | drop | PEG4000 | 11.9 (%) | |
7 | 1 | drop | ammonium sulfate | 0.19 (M) | |
8 | 1 | drop | cobalt hexammine | 90 (mM) | |
9 | 1 | drop | ammonium phosphate | 0.05 (M) |