1BDH
PURINE REPRESSOR MUTANT-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-11-22 |
| Detector | UCSD MARK III |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 176.060, 95.490, 80.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.168 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | UCSD |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Rmerge | 0.067 |
| Number of reflections | 25422 |
| Completeness [%] | 98.0 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.12 (mM) | |
| 10 | 1 | reservoir | PEG4000 | 25 (%) | |
| 11 | 1 | reservoir | ammonium sulphate | 0.4 (M) | |
| 12 | 1 | reservoir | cobalt hexammine | 50 (mM) | |
| 13 | 1 | reservoir | ammonium phosphate | 0.1 (M) | |
| 2 | 1 | drop | dithiothreitol | 0.2 (mM) | |
| 3 | 1 | drop | sodium-potassium phosphate | 38 (mM) | |
| 4 | 1 | drop | DNA | 1.7 (mM) | |
| 5 | 1 | drop | sodium cacodylate | 14.3 (mM) | |
| 6 | 1 | drop | PEG4000 | 11.9 (%) | |
| 7 | 1 | drop | ammonium sulfate | 0.19 (M) | |
| 8 | 1 | drop | cobalt hexammine | 90 (mM) | |
| 9 | 1 | drop | ammonium phosphate | 0.05 (M) |
