1BDF
STRUCTURE OF ESCHERICHIA COLI RNA POLYMERASE ALPHA SUBUNIT N-TERMINAL DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 117.200, 117.200, 350.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.30800 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.740 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.190 |
| Total number of observations | 145903 * | |
| Number of reflections | 27865 | |
| <I/σ(I)> | 11.7 | 2.5 |
| Completeness [%] | 86.9 | 52.3 |
| Redundancy | 5.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.75 | 4 * | pH 8.75 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
| 3 | 1 | reservoir | 0.2 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | PEG400 | 18-22 (%) |
