1BDA
CATALYTIC DOMAIN OF HUMAN SINGLE CHAIN TISSUE PLASMINOGEN ACTIVATOR IN COMPLEX WITH DANSYL-EGR-CMK (DANSYL-GLU-GLY-ARG CHLOROMETHYL KETONE)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.990, 80.780, 159.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 3.350 |
| R-factor | 0.2037 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.745 * |
| Data reduction software | MOSFLM |
| Data scaling software | ROTAGR |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.147 | 0.630 |
| Total number of observations | 27265 * | |
| Number of reflections | 9414 | |
| <I/σ(I)> | 3.6 | 1.2 |
| Completeness [%] | 86.7 | 55.2 |
| Redundancy | 2.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.2 | 23 * | pH 8.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.8 (mg/ml) | |
| 2 | 1 | drop | acetate | 10 (mM) | |
| 3 | 1 | reservoir | acetate | 0.1 (M) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | mPEG6000 | 8 (%) | |
| 6 | 1 | reservoir | methane pentanediol | 4 (%) |
