1BD8

STRUCTURE OF CDK INHIBITOR P19INK4D

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1997-10
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	28.470, 54.560, 45.590
Unit cell angles	90.00, 90.16, 90.00

Refinement procedure

Resolution	? - 1.800
R-factor	0.19 *
Rwork	0.190
R-free	0.26000
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.006 *
RMSD bond angle	0.960 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.800
Rmerge	0.073
Number of reflections	13017
<I/σ(I)>	11.7
Completeness [%]	99.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	4 *	PROTEIN WAS CRYSTALLIZED FROM 35% PEG 4000, 200 MM MGSO4 AND 200 MM TRIS/HCL PH 8.5, SITTING DROP METHOD, AT 4 DEG. C., vapor diffusion - sitting drop, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir		0.2 (M)