1BD7
CIRCULARLY PERMUTED BB2-CRYSTALLIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.000, 48.500, 54.500 |
| Unit cell angles | 66.70, 74.90, 77.50 |
Refinement procedure
| Resolution | 26.880 - 2.780 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BB2 (C-TERMINAL DOMAIN) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.831 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.880 | 2.880 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.071 | 0.081 |
| Number of reflections | 7225 | |
| <I/σ(I)> | 6.6 | 7.7 |
| Completeness [%] | 81.8 | 81.9 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.1 | PROTEIN CONCENTRATION 6 MGS/ML. 0.10 ML OF 0.5M MES-NAOH, PH 6.1, 0.51 ML OF 40% (W/V) PEG 8K, 0.10 ML OF 2M AMMONIUM ACETATE, 0.29 ML OF WATER, AT ROOM-TEMPERATURE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | 0.002ml |
| 2 | 1 | reservoir | PEG8000 | 40 (%) | 0.51ml |
| 3 | 1 | reservoir | ammonium acetate | 2 (M) | 0.1ml |
| 4 | 1 | reservoir | MES | 0.5 (M) | 0.1ml, pH6.1 |
| 5 | 1 | reservoir | water | 0.29ml |
