1BD2

COMPLEX BETWEEN HUMAN T-CELL RECEPTOR B7, VIRAL PEPTIDE (TAX) AND MHC CLASS I MOLECULE HLA-A 0201

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-02-05
Detector	ADSC QUANTUM
Spacegroup name	P 21 21 21
Unit cell lengths	63.800, 73.300, 217.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.238
Rwork	0.238
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1HHI AND 1AO7
RMSD bond length	0.010
RMSD bond angle	27.427 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.110	0.220
Total number of observations	145021 *
Number of reflections	33899
Completeness [%]	91.2	85.1
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.1		12% PEG8000, 20MM MOPS, 100MM MAGNESIUM ACETATE,PH7.1