1BCM
BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-27 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.200, 52.200, 101.600 |
| Unit cell angles | 90.00, 115.50, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.27600 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.320 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.095 |
| Number of reflections | 21089 |
| Completeness [%] | 92.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 13-16 (%(w/v)) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | Na3 citrate | 75 (mM) | |
| 4 | 1 | reservoir | DTT | 2-15 (mM) | |
| 5 | 1 | drop | protain | 16 (mg/ml) |
