1BC7
SERUM RESPONSE FACTOR ACCESSORY PROTEIN 1A (SAP-1)/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.503, 62.306, 38.865 |
| Unit cell angles | 90.00, 107.17, 90.00 |
Refinement procedure
| Resolution | 21.400 - 2.010 |
| R-factor | 0.222 * |
| Rwork | 0.222 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAP-1/E74(DNA) COMPLEX |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.400 | 2.100 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.041 | 0.142 |
| Number of reflections | 10425 | |
| <I/σ(I)> | 21.8 | |
| Completeness [%] | 98.9 | 94 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.8 | 20 * | pH 5.8 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | (NH4)2SO4 | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 25 (mM) | |
| 2 | 1 | drop | ammonium sulfate | 75 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | PEG8000 | 7.0 (%) |
