1BC0
RECOMBINANT RAT ANNEXIN V, W185A MUTANT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1995-07 |
Detector | RIGAKU |
Spacegroup name | H 3 |
Unit cell lengths | 157.300, 157.300, 37.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.29600 |
Structure solution method | DIFFERENCE FOURIER |
RMSD bond length | 0.020 |
RMSD bond angle | 22.585 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.080 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.133 * | |
Number of reflections | 19780 | |
<I/σ(I)> | 20.1 | 1.8 |
Completeness [%] | 86.2 | 58.6 |
Redundancy | 2.6 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.2 | 17 * | PROTEIN CRYSTALLIZED FROM AMMONIUM SULFATE, 20MM CACL2, 50MM HEPPS, PH 8.2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | drop | calcium | 20 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 34-40 (%sat) | |
4 | 1 | reservoir | HEPPS | 50 (mM) | |
5 | 1 | reservoir | 0.02 (%) | ||
6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
7 | 1 | reservoir | 10 (mM) |