1BC0
RECOMBINANT RAT ANNEXIN V, W185A MUTANT
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | RIGAKU |
| Spacegroup name | H 3 |
| Unit cell lengths | 157.300, 157.300, 37.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.29600 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.020 |
| RMSD bond angle | 22.585 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.133 * | |
| Number of reflections | 19780 | |
| <I/σ(I)> | 20.1 | 1.8 |
| Completeness [%] | 86.2 | 58.6 |
| Redundancy | 2.6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.2 | 17 * | PROTEIN CRYSTALLIZED FROM AMMONIUM SULFATE, 20MM CACL2, 50MM HEPPS, PH 8.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | calcium | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 34-40 (%sat) | |
| 4 | 1 | reservoir | HEPPS | 50 (mM) | |
| 5 | 1 | reservoir | 0.02 (%) | ||
| 6 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 7 | 1 | reservoir | 10 (mM) |
