1BBU
LYSYL-TRNA SYNTHETASE (LYSS) COMPLEXED WITH LYSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 181.820, 181.820, 93.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lyl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.840 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.071 | 0.222 |
| Total number of observations | 87419 * | |
| Number of reflections | 25113 | |
| <I/σ(I)> | 6 | 3.2 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 2-mercaptoethanol | 10 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | HEPES | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 46-50 (%sat) | |
| 7 | 1 | reservoir | PEG400 | 2-4 (%) | |
| 8 | 1 | reservoir | glycerol | 15-20 (%) |
