1BBS
X-RAY ANALYSES OF PEPTIDE INHIBITOR COMPLEXES DEFINE THE STRUCTURAL BASIS OF SPECIFICITY FOR HUMAN AND MOUSE RENINS
Experimental procedure
| Spacegroup name | P 21 3 |
| Unit cell lengths | 143.100, 143.100, 143.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.800 |
| R-factor | 0.196 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.025 |
| Refinement software | RESTRAIN |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 * |
| Rmerge | 0.076 * |
| Number of reflections | 22383 * |
| Completeness [%] | 88.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | 0.6 (M) | precipitant | |
| 4 | 1 | reservoir | sodium citrate | 50 (mM) | precipitant |
| 5 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | precipitant |
