1BBJ
CRYSTAL STRUCTURE OF A CHIMERIC FAB' FRAGMENT OF AN ANTIBODY BINDING TUMOUR CELLS
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.500, 93.200, 208.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 * - 3.100 |
R-factor | 0.176 |
RMSD bond length | 0.017 |
RMSD bond angle | 3.730 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
High resolution limit [Å] | 3.100 * |
Rmerge | 0.068 * |
Total number of observations | 57781 * |
Number of reflections | 15644 * |
Completeness [%] | 74.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microdialysis * | 7 * | 4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | Fab' | 10 (mg/ml) | |
2 | 1 | 1 | sodium phosphate | 50 (mM) | |
3 | 1 | 2 | ammonium sulphate | 2.21 (M) | |
4 | 1 | 2 | dioxan | 5 (%(v/v)) | |
5 | 1 | 2 | sodium phosphate | 50 (mM) |