1BBH

ATOMIC STRUCTURE OF A CYTOCHROME C' WITH AN UNUSUAL LIGAND-CONTROLLED DIMER DISSOCIATION AT 1.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	49.200, 56.700, 98.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.185
Rwork	0.185
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	3.300 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.069 *	0.290 *
Total number of observations	102043 *
Number of reflections	25244 *	4102 *
Completeness [%]	96.0 *	95 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *		using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	5 (mg/ml)
2	1	drop	Tris-HCl	0.05 (M)
3	1	drop		0.02 (%(w/v))
4	1	reservoir	PEG4000	25 (%(w/v))