1BB7
LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOBIOSE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-03 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 76.420, 76.420, 54.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.175 * |
| Rwork | 0.175 |
| R-free | 0.24300 |
| Structure solution method | PHASES FOR NATIVE STRUCTURE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.037 |
| Data reduction software | MADNES |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.034 | |
| Total number of observations | 27802 * | |
| Number of reflections | 11498 | |
| <I/σ(I)> | 16.7 | 7.8 |
| Completeness [%] | 91.8 | 83.8 |
| Redundancy | 2.4 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 10 | 277 * | Karlsen, S., (1995) Acta Crystallogr.,Sect.D, 51, 354. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | acetate | 0.1 (M) | |
| 2 | 1 | reservoir | citrate | 0.1 (M) | |
| 3 | 1 | reservoir | phosphate | 0.1 (M) | |
| 4 | 1 | drop | lysozyme | 18 (mg/ml) | 80& of typeI and 20% of type II |
| 5 | 1 | drop | ammonium sulfate | 30-45 (%sat) |
