1BB4

HUMAN LYSOZYME DOUBLE MUTANT A96L, W109H

Experimental procedure

Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-08-20
Detector	X-RAY RESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.050, 64.290, 111.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.000 - 2.230
R-factor	0.249
Rwork	0.249
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lz1
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.000	2.290
High resolution limit [Å]	2.230	2.230
Rmerge	0.101	0.208
Number of reflections	14723
<I/σ(I)>	4.7	3.1
Completeness [%]	96.5	85.1
Redundancy	2.4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		pH 8.5