1BB3

HUMAN LYSOZYME MUTANT A96L

Experimental procedure

Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1996-07-22
Detector	X-RAY RESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	43.660, 65.900, 110.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.188
Rwork	0.188
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lz1
RMSD bond length	0.004
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.820
High resolution limit [Å]	1.790	1.790
Rmerge	0.056	0.026
Number of reflections	30141
<I/σ(I)>	8.4	2.7
Completeness [%]	97.9	89.9
Redundancy	4.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5		pH 4.5