1BAP
A PRO TO GLY MUTATION IN THE HINGE OF THE ARABINOSE-BINDING PROTEIN ENHANCES BINDING AND ALTERS SPECIFICITY: SUGAR-BINDING AND CRYSTALLOGRAPHIC STUDIES
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.460, 71.820, 77.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.750 |
| R-factor | 0.203 * |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.013 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.750 * |
| Total number of observations | 69513 * |
| Number of reflections | 27649 * |
| Completeness [%] | 87.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PEG | 10 (%) | |
| 3 | 1 | drop | citrate | 10 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | sodium azide | 0.02 (%) | |
| 6 | 1 | drop | L-arabinose | 10 (mM) | |
| 7 | 1 | reservoir | PEG | 20 (%) |
