1BAM
STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.400, 46.000, 69.400 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 1.950 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.29700 * |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Number of reflections | 14335 |
| Completeness [%] | 86.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.9 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 10 | 1 | reservoir | PEG8000 | 12 (%) | |
| 11 | 1 | reservoir | 20 (mM) | ||
| 12 | 1 | reservoir | glycerol | 10 (%) | |
| 13 | 1 | reservoir | 0.5 (M) | ||
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | DTT | 1 (mM) | |
| 5 | 1 | drop | glycerol | 15 (%) | |
| 6 | 1 | drop | PEG8000 | 12 (%) | |
| 7 | 1 | drop | 0.25 (M) | ||
| 8 | 1 | drop | 0.2 (%) | ||
| 9 | 1 | drop | crushed crystal |
