1BAJ
HIV-1 CAPSID PROTEIN C-TERMINAL FRAGMENT PLUS GAG P2 DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 61.330, 61.330, 59.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AM3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.056 * | 0.336 * |
| Total number of observations | 19752 * | |
| Number of reflections | 3227 | |
| <I/σ(I)> | 16.2 | 7.9 |
| Completeness [%] | 92.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.35 (mM) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 2-mercaptoethanol | 2 (mM) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | sodium potassium phospahte | 1.6 (M) |
