1BAI

Crystal structure of Rous sarcoma virus protease in complex with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-08-15
Detector	RIGAKU
Spacegroup name	P 65
Unit cell lengths	62.610, 62.610, 132.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.175
Rwork	0.175
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	26.590 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.094
Number of reflections	9601
Completeness [%]	76.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protease	0.23 (mM)
2	1	drop	inhibitor	0.3 (mM)
3	1	reservoir	ammonium sulfate	10 (%sat)