1BAF

2.9 ANGSTROMS RESOLUTION STRUCTURE OF AN ANTI-DINITROPHENYL-SPIN-LABEL MONOCLONAL ANTIBODY FAB FRAGMENT WITH BOUND HAPTEN

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	73.230, 73.230, 373.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.195
Rwork	0.195
RMSD bond length	0.014
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.900 *
Rmerge	0.140 *
Total number of observations	70064 *
Number of reflections	12775 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Fab solution	20 (mg/ml)
2	1	2		150 (mM)
3	1	2		10 (mM)
4	1	2		0.02 (%(w/v))