1B9O

HUMAN ALPHA-LACTALBUMIN, LOW TEMPERATURE FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	286
Detector technology	CCD
Detector	ENRAF-NONIUS
Spacegroup name	P 21 21 21
Unit cell lengths	33.190, 49.550, 64.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.100 - 1.150
R-factor	0.122
R-free	0.16200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	0.014
Data reduction software	MERGEF
Data scaling software	MERGEF
Phasing software	X-PLOR
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.100	1.170
High resolution limit [Å]	1.150	1.150
Rmerge	0.055	0.192
Number of reflections	37696
<I/σ(I)>	10
Completeness [%]	98.0	96
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	4.2		pH 4.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	2 (%)
2	1	1		2 (M)