1B9N
REGULATOR FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.110, 127.360, 62.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.630 - 2.090 |
| Rwork | 0.214 |
| R-free | 0.28600 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.630 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.067 | 0.421 |
| Total number of observations | 268449 * | |
| Number of reflections | 37763 * | |
| <I/σ(I)> | 15.4 | 2 |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 277 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 20 (mM) | ||
| 2 | 1 | reservoir | PEG8000 | 10-18 (%) | |
| 3 | 1 | reservoir | cacodylic acid | 50 (mM) | |
| 4 | 1 | reservoir | magnesium acetate | 100 (mM) |
