1B9M
REGULATOR FROM ESCHERICHIA COLI
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-04 |
Detector | PRINCETON 2K |
Wavelength(s) | 0.8855, 0.9310 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.610, 127.240, 62.990 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.150 - 1.750 |
Rwork | 0.234 |
R-free | 0.27900 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.150 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.055 | 0.368 |
Total number of observations | 205040 * | |
Number of reflections | 53298 * | |
<I/σ(I)> | 31.8 | 2.9 |
Completeness [%] | 80.2 | 66.4 |
Redundancy | 3 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 60 * | 277 * | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 20 (mM) | ||
2 | 1 | reservoir | PEG8000 | 10-18 (%) | |
3 | 1 | reservoir | cacodylic acid | 50 (mM) | |
4 | 1 | reservoir | magnesium acetate | 100 (mM) |