1B9K

ALPHA-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MAR scanner 180 mm plate
Spacegroup name	P 1
Unit cell lengths	39.860, 40.870, 42.470
Unit cell angles	99.40, 95.29, 113.69

Refinement procedure

Resolution	15.550 - 1.900
R-factor	0.169 *
Rwork	0.163
R-free	0.22300 *
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	0.032
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP (SOLOMON)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000 *	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.088	0.295 *
Number of reflections	51805
<I/σ(I)>	6.6	5.3
Completeness [%]	96.9 *	96.9 *
Redundancy	2.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6		20% PEG 4000, 10% ISOPROPANOL, 100MM NA CITRATE PH 6.0, 10MM DTT, 6MG/ML PROTEIN

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir	isopropanol	10 (%)
3	1	reservoir	sodium citrate	100 (mM)
4	1	reservoir	dithiothreitol	10 (mM)