1B9K
ALPHA-ADAPTIN APPENDAGE DOMAIN, FROM CLATHRIN ADAPTOR AP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.860, 40.870, 42.470 |
| Unit cell angles | 99.40, 95.29, 113.69 |
Refinement procedure
| Resolution | 15.550 - 1.900 |
| R-factor | 0.169 * |
| Rwork | 0.163 |
| R-free | 0.22300 * |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.032 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP (SOLOMON) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 * | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | 0.295 * |
| Number of reflections | 51805 | |
| <I/σ(I)> | 6.6 | 5.3 |
| Completeness [%] | 96.9 * | 96.9 * |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 20% PEG 4000, 10% ISOPROPANOL, 100MM NA CITRATE PH 6.0, 10MM DTT, 6MG/ML PROTEIN |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | isopropanol | 10 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 10 (mM) |
