1B9D
MOBILITY OF AN HIV-1 INTEGRASE ACTIVE SITE LOOP IS CORRELATED WITH CATALYTIC ACTIVITY
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-15 |
| Detector | ADSC |
| Wavelength(s) | 1.0162,1.0596 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.350, 72.350, 65.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.033 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.042 * |
| Number of reflections | 20854 |
| Completeness [%] | 99.2 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 15 (%) | |
| 2 | 1 | reservoir | cacodylic acid | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | 5 (mM) | ||
| 6 | 1 | reservoir | 5 (mM) |
