1B9A
PARVALBUMIN (MUTATION;D51A, F102W)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06 |
| Detector | FUJI |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 33.424, 33.424, 298.186 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.2096 * |
| Rwork | 0.210 |
| R-free | 0.29020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpv AUTH A.L.SWAIN R.H.KRETSINGER E.L.AMMA |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.069 |
| Number of reflections | 5432 |
| Completeness [%] | 73.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 50 (mM) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | PEG4000 | 40 (%) |
