1B99
3'-FLUORO-URIDINE DIPHOSPHATE BINDING TO NUCLEOSIDE DIPHOSPHATE KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-15 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.020, 105.310, 70.350 |
| Unit cell angles | 90.00, 117.43, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.700 |
| Rwork | 0.207 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lwx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.092 |
| Number of reflections | 19650 * |
| Completeness [%] | 81.0 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | tris | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NDPK |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | nucleotide | 15 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | 20 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 16 (%) |
