1B99
3'-FLUORO-URIDINE DIPHOSPHATE BINDING TO NUCLEOSIDE DIPHOSPHATE KINASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 278 |
Detector technology | IMAGE PLATE |
Collection date | 1997-01-15 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.020, 105.310, 70.350 |
Unit cell angles | 90.00, 117.43, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
Rwork | 0.207 |
R-free | 0.30400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lwx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.250 * |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.092 |
Number of reflections | 19650 * |
Completeness [%] | 81.0 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 1 | tris | ||
3 | 1 | 1 | MGCL2 | ||
4 | 1 | 1 | NDPK |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | reservoir | nucleotide | 15 (mM) | |
3 | 1 | reservoir | Tris-HCl | 50 (mM) | |
4 | 1 | reservoir | 20 (mM) | ||
5 | 1 | reservoir | PEG6000 | 16 (%) |