1B8S
CHOLESTEROL OXIDASE FROM STREPTOMYCES GLU361GLN MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.300, 73.390, 63.180 |
| Unit cell angles | 90.00, 104.98, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.19 * |
| Rwork | 0.190 |
| R-free | 0.23100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.050 | 0.183 |
| Total number of observations | 281106 * | |
| Number of reflections | 49835 | |
| <I/σ(I)> | 35.1 | 2 |
| Completeness [%] | 91.8 | 52.5 |
| Redundancy | 3.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 17 * | PRECIPITANT CONDITIONS: 10-12% PEG 8000, 100MM SODIUM CACODYLATE PH 5.2, 75MM MNSO4 PROTEIN CONCENTRATION 8.5MG/ML , VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 10-12 (%(w/v)) | |
| 4 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 5 | 1 | reservoir | 75 (mM) |
