1B8J
ALKALINE PHOSPHATASE COMPLEXED WITH VANADATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-05 |
| Detector | SDMS |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 195.410, 167.810, 76.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.177 * |
| Rwork | 0.177 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1alk |
| RMSD bond length | 0.019 |
| RMSD bond angle | 24.700 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR (3.1 (MSI)) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.420 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.900 |
| Rmerge | 0.064 | 0.390 |
| Total number of observations | 266977 * | |
| Number of reflections | 93722 | |
| <I/σ(I)> | 6.6 | 0.92 |
| Completeness [%] | 94.6 | 85.3 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.5 * | CRYSTALLIZATION CONDITIONS: 65 % SATURATING (NH4)2SO4, 100 MM TRIS, 10 MM MGCL2, 1 MM ZNCL2, 0.1 MM NH4VO3, PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 30 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 20 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 100 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 0.01 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 36-39 (%sat) |
