1B8I

STRUCTURE OF THE HOMEOTIC UBX/EXD/DNA TERNARY COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	113
Detector technology	CCD
Collection date	1998-12-15
Detector	ADSC
Spacegroup name	P 43 21 2
Unit cell lengths	69.430, 69.430, 100.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.224 *
Rwork	0.224
R-free	0.30400
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	21.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.074 *	0.185 *
Total number of observations	110271 *
Number of reflections	10324
Completeness [%]	98.3	100
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.5		0.6M AMMONIUM PHOSPHATE, pH 4.5, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium phosphate	0.6 (M)