1B8G
1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-20 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.630, 69.060, 123.240 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 19.700 - 2.370 |
| Rwork | 0.179 |
| R-free | 0.24200 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.700 | 2.430 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.098 * | 0.104 * |
| Number of reflections | 34176 | |
| <I/σ(I)> | 9.8 | 6.2 |
| Completeness [%] | 93.6 | 80.8 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 0.2 (mM) | |
| 3 | 1 | drop | PLP | 0.01 (mM) | |
| 4 | 1 | drop | 0.01 (%(w/v)) | ||
| 5 | 1 | drop | HEPES | 30 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 18-24 (%(w/v)) | |
| 7 | 1 | reservoir | MES | 50 (mM) |
