1B8C
PARVALBUMIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.231, 50.211, 55.374 |
| Unit cell angles | 90.00, 99.22, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.1967 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpv AUTH A.L.SWAIN R.H.KRETSINGER E.L.AMMA |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.014 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.085 |
| Number of reflections | 13158 |
| Completeness [%] | 95.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | CRYSTALLIZATION CONDITIONS: 40% PEG 4000, 200 MM MGCL2, 50 M PH 7. THE CRYSTALS WERE GROWN AT 4 DEGREES C., pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 50 (mM) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | PEG4000 | 40 (%) |
