1B87
CRYSTAL STRUCTURE OF AN AMINOGLYCOSIDE 6'-N-ACETYLTRANSFERASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 1998-01-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9809, 0.9795, 0.9792, 0.9770 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 146.960, 146.960, 146.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| Rwork | 0.188 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.940 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.127 * | 0.332 * |
| Number of reflections | 6085 | |
| <I/σ(I)> | 4.3 | 1.4 |
| Completeness [%] | 89.7 | 88.9 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 22 * | pH 7.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | D(+)-SUCROSE | ||
| 2 | 1 | 1 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7-10 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | D(+)-sucrose | 5 (%(w/v)) | |
| 4 | 1 | drop | ammonium sulfate | 0.7 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
