1B86
HUMAN DEOXYHAEMOGLOBIN-2,3-DIPHOSPHOGLYCERATE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Temperature [K] | 293 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.300, 98.080, 65.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| Structure solution method | OTHER |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.025 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.098 |
| Number of reflections | 28482 |
| Completeness [%] | 84.0 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.2 | 20 * | pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG1000 | 18-25 (%(v/v)) | |
| 2 | 1 | 1 | sodium phosphate | ||
| 3 | 1 | 1 | protein | 30-50 (mg/ml) |
