1B7Y
PHENYLALANYL TRNA SYNTHETASE COMPLEXED WITH PHENYLALANINYL-ADENYLATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-15 |
| Detector | RIGAKU |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 174.500, 174.500, 140.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.230 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pys |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.064 | 0.399 |
| Number of reflections | 81613 | |
| <I/σ(I)> | 6 | 2.2 |
| Completeness [%] | 95.7 | 88.5 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 4 * | Chernaya, M., (1987) J. Mol. Biol., 198, 555. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | IMIDASOL-HCL | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | NAN3 | ||
| 4 | 1 | 1 | (NH4)2SO4 | ||
| 5 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-5 (mg/ml) | |
| 2 | 1 | drop | imidazole-HCl | 20 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | ammonium sulfate | 15 (%sat) | |
| 6 | 1 | reservoir | ammonium sulfate | 25-30 (%sat) | |
| 7 | 1 | reservoir | imidazole-HCl | 20 (mM) |
