1B7S
VERIFICATION OF SPMP USING MUTANT HUMAN LYSOZYMES
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-10 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.490, 62.010, 32.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| Structure solution method | ISOMORPHOUS METHOD |
| Starting model (for MR) | WILD-TYPE OF HUMAN LYSOZYME |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.640 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | |
| Total number of observations | 21097 * | |
| Number of reflections | 7151 * | |
| Completeness [%] | 88.8 | 81.2 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | 10 * | Takano, K., (1995) J.Mol.Biol., 254, 62. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 2.5 (M) | ||
| 3 | 1 | reservoir | acetate | 20 (mM) |
