1B7F
SXL-LETHAL PROTEIN/RNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.900, 86.800, 160.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.201 * |
| Rwork | 0.201 |
| R-free | 0.29400 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.649 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.089 * | 0.411 * |
| Total number of observations | 98612 * | |
| Number of reflections | 17053 | |
| <I/σ(I)> | 12.03 | 1.62 |
| Completeness [%] | 98.5 | 87.4 |
| Redundancy | 5.8 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | pH 7.0, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | NA HEPES | ||
| 3 | 1 | 1 | LI2SO4 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | DIOXANE | ||
| 6 | 1 | 1 | ZINC ACETATE | ||
| 7 | 1 | 1 | MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 10 (%) | |
| 3 | 1 | reservoir | sodium MOPS | 50 (mM) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | spermine | 3 (mM) | |
| 6 | 1 | reservoir | dioxane | 1-3 (%) | |
| 7 | 1 | reservoir | zinc acetate | 2 (mM) | |
| 8 | 1 | reservoir | 2 (mM) |
